Journal: Signal Transduction and Targeted Therapy

Article Title: Abrogation of aberrant glycolytic interactions eliminates senescent cells and alleviates aging-related dysfunctions

doi: 10.1038/s41392-025-02502-6

Figure Lengend Snippet: A PGAM1-Chk1 antagonist suppresses FOXM1 during senescence. a , b Assessment of the proapoptotic BH family in SnCs after Nutlin 3b treatment. a RT‒PCR analysis of SnCs with or without Nutlin 3b. ( n = 3, biological replicates). b p53 siRNA-transfected SnCs were exposed to Nutlin 3b. RT‒PCR analysis of the indicated cells. c Knockdown of BIM in SnCs. SnCs were transfected with the indicated siRNA, siBIM, or scramble control RNA. The transfectants were then subjected to Nutlin 3b treatment. d – f Comparison of the results of the microarray analysis between the control and Nutlin 3b-treated SnCs ( n = 3, biological replicates). d Fold enrichment analysis by comparison between 1168 genes downregulated by Nutlin 3b in SnCs and 9442 datasets of chromatin immunoprecipitation (ChIP) information reported in the National Center for Biotechnology Information (NCBI), European Bioinformatics Institute (EBI) and DNA Data Bank of Japan (DDBJ). Eighty-six of the 9442 datasets displayed high enrichment (>3-fold). Twenty-one datasets for FOXM1 are shown in red (left panel). e Heatmap of 37 FOXM1 target genes downregulated by Nutlin 3b. SnCs with or without Nutlin 3b were compared. f Gene expression of the FOX family in SnCs after Nutlin 3b treatment, as determined via microarray data. Left panel, heatmap analysis of the FOX family. Right panel: Red and blue bars indicate reduced and increased expression, respectively. Dagger (†) indicates |FC|≥2 and Lpe. P < 0.05 is presented in Supplementary Fig. . Evaluation of FOXM1 protein levels in SnCs after Nutlin 3b treatment ( g ) and after transfection with HIF-2α siRNA ( h ). The data are representative of two independent experiments. i Evaluation of BIM mRNA in SnCs after FOXM1 knockdown. FOXM1 siRNA was transfected into SnCs ( n = 3, biological replicates). j Schematic diagram of the promoter region in the human BIM gene. Several cis-elements for FKH are shown. The indicated regions were subsequently cloned and inserted into luciferase reporter plasmids (left lower panel). Luciferase reporter assays were performed in SnCs after Nutlin 3b treatment (middle panel). A reporter assay for the proximal region was conducted in SnCs after FOXM1 knockdown (right panel) ( n = 3, biological replicates). The data represent the means ± SEMs. Single (*) and double (**) asterisks indicate statistical significance at p < 0.05 and p < 0.01, respectively. Statistical analyses were performed via unpaired Student’s two-tailed t test ( a ) or one-way analysis of variance (ANOVA) and Dunnett’s multiple comparison test ( b , c , i and j )

Article Snippet: Anti-mouse Chk1 (25887-1-AP, 1:1000) and anti-mouse FOXM1 (13147-1-AP, 1:1000) were purchased from Proteintech (USA).

Techniques: Transfection, Knockdown, Control, Comparison, Microarray, Chromatin Immunoprecipitation, Gene Expression, Expressing, Clone Assay, Luciferase, Reporter Assay, Two Tailed Test

Journal: Signal Transduction and Targeted Therapy

Article Title: Abrogation of aberrant glycolytic interactions eliminates senescent cells and alleviates aging-related dysfunctions

doi: 10.1038/s41392-025-02502-6

Figure Lengend Snippet: In vivo FOXM1 accumulation in aged tissues is targeted by Nutlin 3b. a In vivo profiles of FOXM1 mRNA in several tissues of 4-, 10-, 40- and 90-week-old mice, in addition to embryos on day 13.5. The indicated tissues were collected for RNA extraction. b Fold enrichment analysis comparing upregulated genes in 90-week-old mice (liver: 1039; kidney: 1882; lung: 386; and WAT: 385) and ChIP datasets (liver: 9649; kidney: 11,195; lung: 6147; and WAT: 6059). Comparative transcriptomic analysis of the indicated tissues, liver, lung, kidney, and WAT, was performed between 10-week-old and 90-week-old mice. c Heatmap analysis of FOXM1 target genes in the kidney and lung (left and right panels) of young (10-week-old) and aged (90-week-old) mice. d Assessment of FOXM1 target genes involved in the cell cycle and DNA repair genes in aged kidneys and lungs (upper and lower panels, respectively) after Nutlin 3b treatment via RT‒PCR analysis. Tissues were collected from young (10-week-old), aged (90-week-old) and Nutlin 3b-treated aged mice. e The impact of Nutlin-3b on aged kidneys. Representative pictures of the glomerulus after Nutlin 3b treatment (upper panels). Samples from young, aged and Nutlin 3b-treated aged mice. The bar indicates 50 μm. Hyalinosis scores were assessed in aged mice with or without Nutlin 3b treatment. Glomerulus region (lower left) and interstitial region (lower right). f Immunohistochemical examination of the lung for p21 CIP1 . Representative images of stained samples from the indicated mice are shown (left panels). The bar indicates 20 μm. The ratio of p21 CIP1 -positive cells was assessed (right panel). g Comparison of the mRNA levels of p16 Ink4a and SASP factors (Il6, Tnfα, Cxcl1, and Ccl5). Lung extracts from the indicated mice were analyzed via RT‒PCR. The data represent the means ± SEMs. Single (*) and double (**) asterisks indicate statistical significance at p < 0.05 and p < 0.01, respectively. Statistical analyses were performed via one-way analysis of variance (ANOVA) and Dunnett’s multiple comparison test

Article Snippet: Anti-mouse Chk1 (25887-1-AP, 1:1000) and anti-mouse FOXM1 (13147-1-AP, 1:1000) were purchased from Proteintech (USA).

Techniques: In Vivo, RNA Extraction, Immunohistochemical staining, Staining, Comparison

Journal: Cancer Cell International

Article Title: Icariin-curcumol inhibits histone H3K18 lactylation and FOXM1 expression to enhance the sensitivity of prostate cancer cells to docetaxel

doi: 10.1186/s12935-025-03927-3

Figure Lengend Snippet: Histone lactylation regulates FOXM1 expression in LNCaP/R cells. A ChIP-qPCR analysis of H3K18la enrichment in the FOXM1 promoter region after DTX treatment. *** P < 0.001 vs. LNCaP. ### P < 0.001 vs. LNCaP/R. B ChIP-qPCR analysis of H3K18la enrichment in the FOXM1 promoter region after Nala or 2-DG treatment. C Dual-LUC analysis of FOXM1 promoter activity after Nala or 2-DG treatment. D - E . qRT-PCR D and western blot E of FOXM1 mRNA expression and protein levels in LNCaP/R cells after Nala or 2-DG treatment. * P < 0.05; ** P < 0.01; *** P < 0.001 vs. LNCaP/R

Article Snippet: FOXM1 , 13147-1-AP, Proteintech , Rabbit , 1:2000.

Techniques: Expressing, ChIP-qPCR, Activity Assay, Quantitative RT-PCR, Western Blot

Journal: Cancer Cell International

Article Title: Icariin-curcumol inhibits histone H3K18 lactylation and FOXM1 expression to enhance the sensitivity of prostate cancer cells to docetaxel

doi: 10.1186/s12935-025-03927-3

Figure Lengend Snippet: FOXM1 modulates proliferation and lactylation in LNCaP/R cells. A qRT-PCR and western blot of FOXM1 silencing efficiency in LNCaP/R cells. B Western blot analysis of H3K18la levels in LNCaP/R cells. C LDH level quantificationin LNCaP/R cells. D CCK8 assay for viability. E EdU staining to assess proliferation. Scale bar = 50 μm. F Apoptosis analysis by flow cytometry. * P < 0.05; ** P < 0.01; *** P < 0.001 vs. LNCaP/R + DTX + sh-NC

Article Snippet: FOXM1 , 13147-1-AP, Proteintech , Rabbit , 1:2000.

Techniques: Quantitative RT-PCR, Western Blot, CCK-8 Assay, Staining, Flow Cytometry

Journal: Cancer Cell International

Article Title: Icariin-curcumol inhibits histone H3K18 lactylation and FOXM1 expression to enhance the sensitivity of prostate cancer cells to docetaxel

doi: 10.1186/s12935-025-03927-3

Figure Lengend Snippet: Ica-Cur and histone lactylation regulate DTX sensitivity in vivo. A Xenograft tumors in BALB/c nude mice 35 days after subcutaneous injection of LNCaP/R cells. B Tumor volume calculated as 0.5 × length × width 2 . C Tumor weight at day 35. D Body weight monitoring. E IF staining of pan-Kla, H3K18la, and LDHA in tumor sections. Scale bar = 25 μm. F Western blot analysis of pan-Kla in tumor tissues. G qRT-PCR and western blot of FOXM1 mRNA and protein levels in tumors. * P < 0.05; ** P < 0.01; *** P < 0.001 vs. LNCaP/R. &&& P < 0.001 vs. Nala. # P < 0.05; ## P < 0.01; ### P < 0.001 vs. Ica-Cur

Article Snippet: FOXM1 , 13147-1-AP, Proteintech , Rabbit , 1:2000.

Techniques: In Vivo, Injection, Staining, Western Blot, Quantitative RT-PCR